The article deals with the problem of microorganisms’ resistаnce to antibiotics at dairy farms and complexes (DFC). The aim of the work – analysis of resistance of E. coli to antibiotics used at DFC. As a result we determined the range of resistance of coli-forms to antibiotics used at the dairy complex “Zabolot” of Grodno district. It was shown that received strains of microorganisms E. coli have high resistance to the following antibiotics: oletetrin, erythromycin, polymyxin, benzylpenicillin. E. coli bacteria remained high sensitivity to ceftriaxon, sisomicin, furadonin, kanamycin, gentamicin. This fact allows to recommend these antibiotics for animals coli-bacteriosis treatment at DFC.