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Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase
Chastukhina I., Sharipova M., Gabdrakhmanova L., Balaban N., Kostrov S., Rudenskaya G., Leshchinskaya I.
and growth retardation phase. Proteinase accumulation was activated by complex organic substrates (casein
Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase
Chastukhina I., Sharipova M., Gabdrakhmanova L., Balaban N., Kostrov S., Rudenskaya G., Leshchinskaya I.
and the growth retardation phase. Proteinase accumulation was activated by complex organic substrates (casein
Optimized medium for the efficient production of bacillus intermedium glutamyl endopeptidase by the recombinant bacillus subtilis
Gabdrakhmanova L., Shakirov E., Balaban N., Sharipova M., Rudenskaya G.
, organic and inorganic nitrogen sources, and yeast extract. Complex protein substrates, such as casein
Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius
Balaban N., Gabdrakhmanova L., Sharipova M., Sokolova E., Malikova L., Mardanova A., Rudenskaya G., Leshchinskaya I.
not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production
Effect of nutrients on the accumulation of glutamyl endopeptidase in the culture liquid of bacillus intermedium 3-19
Shakirov E., Gabdrakhmanova L., Balaban N., Sharipova M., Rudenskaya G., Leshchinskaya I.
cations enhanced it. Protein substrates, such as casein, gelatin, and hemoglobin, did not affect enzyme
Optimized medium for the efficient production of Bacillus intermedium glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73
Gabdrakhmanova L., Shakirov E., Balaban N., Sharipova M., Rudenskaya G., Kostrov S., Akimkina T., Leshchinskaya I.
, organic and inorganic nitrogen sources, and yeast extract. Complex protein substrates, such as casein
Effect of nutrients on the accumulation of glutamyl endopeptidase in the culture liquid of Bacillus intermedius 3-19
Shakirov E., Gabdrakhmanova L., Balaban N., Sharipova M., Rudenskaya G., Leshchinskaya I.
cations enhanced it. Protein substrates, such as casein, gelatin, and hemoglobin, did not affect enzyme
The cellular location of proteolytic enzymes of Bacillus intermedius
Sharipova M., Shakirov E., Balaban N., Gabdrakhmanova L., Shilova M., Rudenskaya G., Leshchinskaya I.
fluid. Proteolytic activity towards casein was also detected in the cytoplasm. The proteinases localized
Development of modification technology of wood raw material in production of solid biofuel
Sychevа, N. A., Khmyzov, I. A., Molchan, A. V., Beloded, T. A., Solov’yeva, T. V.
by the addition of protein nature (albumin, casein , gelatin) and starch (potato starch and maize, starch syrup
Glutamyl endopeptidase of Bacillus intermedius, strain 3-19
Leshchinskaya I., Shakirov E., Itskovitch E., Balaban N., Mardanova A., Sharipova M., Viryasov M., Rudenskaya G., Stepanov V.
H optima at pH 7.5 and 9.0 for casein and one at pH 8.0 for Z-Glu-pNA hydrolysis. A 6 mM K(m) is found

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